Free radicals and reactive oxygen species (ROS) are highly reactive molecules that are generated by normal cellular processes, environmental stress, and UV irradiation. ROS react with cellular components,

Use of human samples including peripheral blood mononuclear cells (PBMCs) in drug discovery is critical for increasing the chances of success for a small molecule screen hit. A review based on recent

The influenza season in Europe is over... until next year. Influenza remains one of the most important public healthcare problems, especially in risk groups.

Lipodystrophies are disorders characterized by complete or selective loss of adipose tissue from various regions of the body. They might lead to severe metabolic disorders. The development of reliable

Mechanisms leading to cellulite formation is complex. It involves lipid regulatory pathways and proinflammatory cross-talk that represent promising molecular targets in cosmetology. This post introduces

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

Oxidative stress can lead to diverse pathophysiological changes in the body. Neurodegenerative diseases such as Parkinson and Alzheimer are linked to oxidative

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System