Page 9 - Scientific Library

Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such

Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.

This technology produces first a targeted break in genomic DNA, which can then be exploited to produce

Biomarkers specialists are often asked to select an ELISA kit for researchers: with thousands of ELISA references available on the market, the choice can be tricky regarding proteins for which several

Nucleosomes are basis units of DNA packaging in eukaryotic cells. A core particle (mono-nucleosome) consists of a segment of DNA called core DNA (147 bp in length)

Interferons (IFNs) are low molecular weight proteins that belong to the class of glycoproteins known as cytokines. IFNs are part of the non-specific immune system and are an important first line of defense

In previous posts, we have discussed about whether samples should be pooled, how much (and if) they should be diluted, and how biologicals can affect the final results...and the biomarkers that you find.

CRISPR/Cas9 is relatively simple to implement, as the researcher fully controls the experimental design of the tools, from the sgRNA sequence to the Cas9 protein.

A recent article by E. Langer (E. Langer, “Another In-House Operation Gets Outsourced” (2015) Pharmaceutical Technology 39 (6)) describes the growing

The role of STAT3 signaling in the activity of dendritic cells (DC) has been recently revealed in a publication by Okada et al.

For secretome biomarker profiling, several solutions, including arrays allowing to screen up to 1,000 factors, have been available for some time now. These profiling solutions, however, were semi-quantitative

A publication earlier this year by Bárcena et al. shows that angiogenin plays a crucial role in the onset and development of liver cancer (hepatocellular carcinoma or HCC).

Angiogenin was the first