Page 3 - Scientific Library
Tips and Tricks: how to increase unstable protein expression in E. Coli
Recombinant protein expression and purification for therapeutic development or for in vitro studies can sometimes be a real challenge. It requires huge investments in both time and cost in order to obtain
Why you should outsource your production of proteins - both stable or unstable
Whether your protein is simple to produce, or complex and requires more development, in any case, outsourcing your protein expression, purification and production is a very viable option for your projects.
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
Achieve success with RNA-based CRISPR-CAS9 knock-out
RNA-based CRISPR-CAS9 gene editing is a vector-free approach that is required for therapeutic perspectives. However, from a practical point of view, what appears as a major challenge to engineer primary
How to produce high yield capped mRNA
Messenger RNA can be used for protein expression into the cells. The delivery of mRNA is the delivery of a new function that can activate a biological process. In the particular case of the CAS9 mRNA,
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient
shRNA set with improved performances
Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the
Hyalose Select-Hyaluronic Acidâ„¢ products now available now from tebu-bio
Echelon Biosciences are specialized in the development of novel assays and reagents in Lipid research, with the commitment to innovate and provide the best solutions for researchers. They have recently
Optogenetic tools: easily control cAMP production in cells with Light
Non-invasive manipulations of intracellular processes have recently appeared as an emerging research field. Experts in the development of biosensors for live cell discovery, Montana Molecular provide an
MitoTools: Mitochondrial research has never been so easy
Involved in many physiological processes (apoptosis, aging, cell signalling..) and numerous diseases (cardiovascular disorders, to cancer and neuro-degenerative diseases…), Mitochondria have become more
ORF expressing Lentiviral system
ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell