Page 3 - Scientific Library

DNA and Histone proteins are targets for epigenetic modifications. While DNA is only methylated, histones can be targets for diverse modifications such as methylation, acetylation, ubiquitination, phosphorylation

Free radicals and reactive oxygen species (ROS) are highly reactive molecules that are generated by normal cellular processes, environmental stress, and UV irradiation. ROS react with cellular components,

Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so there shouldn't be any need to look for alternatives

What are metabolites?

Microorganisms live in a world of chemical signals. They use small molecular weight compounds, known as metabolites, to regulate their own growth and development, to encourage other

Research

Methylation of DNA plays a crucial role in transcriptional regulation, imprinting, and chromatin

Nucleosomes are basis units of DNA packaging in eukaryotic cells. A core particle (mono-nucleosome) consists of a segment of DNA called core DNA (147 bp in length)

Isolation of highly purified untagged proteins is crucial in today's R&D programs. Up to now, recombinant protein production approaches were often based on the use of "tags" to make protein purification

Pharmaceutical, biotech and medical device industries apply restrictive quality systems in their bio-production processes. Among the numerous parameters controlled daily by scientists, I have selected

SUMO-tag gene fusion acts as both a chaperonin and as an initiator of protein folding in both prokaryotic and eukaryotic systems. Addition of this tag enables protein experts to increase the expression

In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.

Tip #1

Traditional methods to detect and purify poly-ubiquitinated proteins require either anti-ubiquitin antibodies or Ubiquitin Binding Associated domains (UBAs). Most of the current methods display rather