Page 3 - Scientific Library
Obesity (adipose tissue) and Diabetes - which ELISA tests, arrays and cellular models?
If your research is related to obesity and adipose tissue, you may be interested by the selection I have performed below of immunoassays (including ELISA tests and antibody arrays), as
New Reporter cell lines for Drug discovery and Immunotherapy checkpoint research
In the last few decades, in the field of Drug Discovery, Immunotherapy has gained more and more importance for the treatment of various diseases and in particular for certain types of
ELISA test to confirm elimination of Host Cell Proteins (HCP) from bioproductions
Expression systems - removing their contaminants
CHO, E. Coli and Pichia are useful expression systems for drug production. This can be for proteins, enzymes or antibody productions. Research and
How can you improve your reporter gene assays?
Gene reporter assays are widely used in research and Drug Discovery. The development of such cell lines is often time-consuming and costly, sometimes upwards of 5-20k € per cell line.
Immunotherapy research: Easily build your own reporter cell line
As discussed previously in our recent Drug Discovery post, Immunotherapy is one of the central research fields for various disease treatments and also certain cancers. A major regulator
6 ELISA test formats for protein target and biomarker discoveries
Having access to highly reproducible and fully validated ELISA tests is crucial in today's Life Science research. Although the Sandwich ELISA format is the most commonly used, other immunoassay
How do you choose the best ELISA test for your project ?
There are many, many ELISA tests out there to choose from, and making the best selection can sometimes be a bit daunting. In this post, let's take a look at the key points to keep in mind.
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha
How to easily screen adenosine A2AR agonists and antagonists
Recently, BPS Bioscience have released a new Adenosine A2A Receptor (A2AR / ADORA2A) Functional Recombinant Cell Line for screening A2AR agonists and antagonists. An excellent means to
Recombinant CYP Bactosomes: versatile formats for many ADME applications
Recombinant CYPs are commonly used in ADME-Tox studies as in vitro tools, mostly for reaction phenotyping, enzyme inhibition screening, clearance and metabolite ID.
"Research
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily