Page 6 - Scientific Library
Tips and Tricks: how to increase unstable protein expression in E. Coli
Recombinant protein expression and purification for therapeutic development or for in vitro studies can sometimes be a real challenge. It requires huge investments in both time and cost in order to obtain
New one-step cGMP immunoassay with improved sensitivity
When scientists at ArborAssays invented the Direct Cyclic Nucleotide EIA immunoassay more than 20 years ago, they knew the kits would be popular. The direct nature of the format for the assays allows cAMP
Immunoassays with collagen: Tips to optimize anti-collagen antibody performance
Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary
Tips to select the best secondary
When doing WBs, IFs or IHCs, we normally pay a lot of attention to the primary antibody that should be chosen. We look at the datasheet, we check that the primary antibody has been validated for the species
Monoclonal antibodies - all you need to know about antibody generation
Antibody generation raises a number of questions... what is the difference between their classes, forms and types? And how do recombinant monoclonal antibodies (rAbs) overcome the drawbacks of classical
Gold standard one component TMB
Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to blue reveals the activity
How to obtain reliable DNA and RNA quantitation
The iQuant kits developped by ABP Bioprobes provide accurate quantitation of DNA and RNA with a wide range including high sensitivity. They are useful for NGS applications, qPCR analysis, and in vitro
Hepatic Hyaluronan Content increased by Alcohol Consumption in Patients with Liver Steatosis
In a previous post, I introduced Sekisui Xenotech's Research Biobank, which is based on rejections from liver transplantation as opposed to post-mortem tissues, thus offering a guarantee for better tissue
Study 10 cytokines and more for the price and simplicity of a single ELISA
I just want to briefly outline in this post the power of antibody microarray when it comes to time and cost saving as well as versatility. Whether you want to do multiple profiling or quantitative studies,
Mass Spectrometry & Antibody Arrays in Biomarker Research: a complementary approach
When it comes to proteomics and broad protein profiling (and/or quantification), two main technologies come to mind: Mass Spectrometry and Antibody Arrays. Rather than opposing each other, they appear
PROTACs: A promising strategy in Drug Development
Thanks to its capability to recruit the natural protein degradation system of a cell, PROTACs (PROteolysis TArgeting Chimera) appears as a very promising technology in cancer therapy and disease treatment.
Multiplex Protein Assays: which one fits you best?
Multiplex protein assays allow multiple targets quantification in a single sample, a bit like performing up to 40 different ELISAs in one go. Outsourcing Multiplex Protein Quantification projects to experts