Page 3 - Scientific Library
5 new research tools to perform chromatin and epigenetic analysis
Nucleosomes are core particles involved in DNA packaging in eukaryotic cells. They affect gene expression levels by regulating access of DNA to transcription factors and DNA binding proteins. Nucleosome
Site-specific modified-histone substrates
Recombinant Histone proteins are popular research solutions in Drug discovery and Epigenetics studies. They can be used as in vitro positive controls in the analysis of post-translational Histone
How to probe motor-domains of kinesins?
In the past, molecular motor proteins like kinesins have been investigated with macromolecular approaches. Recent research on kinesins has been focused on resolving how kinesin is regulated by intramolecular
Growth factors in cartilage biology and osteoarthritis
Cartilage is a flexible connective tissue which can rapidly grow and which is specialized to absorb and resist compression. The perichondrium, a vascularized layer, surrounds the tissue which does not
Epigenetics Writers - Readers - Erasers: Targeting Readers in Drug Discovery
DNA and Histone proteins are targets for epigenetic modifications. While DNA is only methylated, histones can be targets for diverse modifications such as methylation, acetylation, ubiquitination, phosphorylation
Immunotherapy Screening - GITR:GITRL pathway
In previous blogs, I invited you to read about the relevance of the B7-1
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up with the
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
E3 Ligases: RING- or HECT-mediated ubiquitination?
A large number of cellular processes are regulated by the reversible conjugation of Ubiquitin (UB) proteins to substrates. UB-related research tools (including E1-, E2 and E3 Ligases) are now commercially available
Kit-Free Site Directed Mutagenesis Protocol
Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol