Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

Signal-Seeker assays (Cytoskeleton Inc.) provide researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs)

Small GTP-binding proteins such as Rho and Ras GTPase family members are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. In this

The ubiquitin-proteasome system (UPS) is a well-characterized protein degradation system in cells whose dysfunction is implicated in many diseases, including neurodegeneration and cancer^1,2. Major UPS

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras GTPase superfamily. Due to their role as molecular switches in cell signalling (active GTP-bound state

As is well known, the actin cytoskeleton is a highly dynamic structure, involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis,

My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing

Neuronal polarity is essential for the proper development, growth, and physiology of neurons. Within neurons, microtubules (MTs) and motor protein polarities are required for establishing and maintaining

We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we've established

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System