Scientific Library

Gene expression is regulated by different mechanisms. One of them is the binding of

In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to

In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated

Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers

Non-invasive manipulations of intracellular processes have recently appeared as an emerging research field. Experts in the development of biosensors for live cell discovery, Montana Molecular