Scientific Library
Tips for Optimizing Cell Culture Conditions with Hydrogels
In a previous post on Cellular Matrices for Translational Research, I introduced HyStem hydrogels. In order to engineer a custom culture system with HyStem, simple pilot studies or optimization
Ready-to-Use ELISAs to study Transcription Factors DNA binding
Gene expression is regulated by different mechanisms. One of them is the binding of Transcription
Biomolecule conjugation - how to biotinylate with reproducible results?
Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low efficiency.
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based
Improve MS-based biomarker determination with immunocapture
During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers
Recent developments in 3D cell culture hydrogel
Due to their ability to simulate the characteristics of most soft tissues and natural scaffolds, hydrogels are now an attractive material for developing synthetic Extracellular Matrix (ECM) analogs.
Recently,