Immunoediting of cytokine signals provides another area of pathways which tumours utilise to evade and potentially escape from immunological targeting. This post aims at shedding some light into the

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Understanding how mammalian cells function requires a dynamic perspective. Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

As with this painted representation of (surrealist!) DNA, to a certain extent, molecular cloning is an art.

Unfortunately for newcomers, or fortunately for experts, it's required for many downstream applications

Inflammation is involved in many fields including cosmetology, drug discovery (including RNA-based vaccines), and diagnostic index development, as well as being linked to almost every disease such as HIV,

In a recently published study, researchers sought to elucidate if 2 treatments modulated immune response or not, despite the available evidence being conflicting. The method that made the difference and

One of the challenges in R&D is to find robust technologies to develop diagnostic tests. Notably, it could be within a context of asymptomatic infections for which reliable detection of positive specimens

I just want to briefly outline in this post the power of antibody microarray when it comes to time and cost saving as well as versatility. Whether you want to do multiple profiling or quantitative studies,

The close relation between Severe Acute Respiratory Syndrome (SARS), unpaired adaptive immune responses and overexpression of inflammatory cytokines has been described more than a decade ago (1, 2).
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Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System