Scientific Library

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras GTPase superfamily. Due to their role as molecular switches in cell signalling (active GTP-bound state

As is well known, the actin cytoskeleton is a highly dynamic structure, involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis,

Neuronal polarity is essential for the proper development, growth, and physiology of neurons. Within neurons, microtubules (MTs) and motor protein polarities are required for establishing and maintaining

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of

Following on from the popular SiR-Probes, earlier this year Spirochrome introduced new SPY probes, as presented in this post.

Take a few minues to watch this video of the SPY™ Actin, Tubulin and DNA

Despite its importance in the regulation of many cellular processes, membrane tension is still complicated to measure. And despite valuable insights, the techniques currently available remain complex and

With the great success of the SIR-probes for efficient labeling of the cell Cytoskeleton, DNA and Lysosome, Spirochrome have just launched a series of 10 new live cell fluorescent tools : The SPY^TM probes