Scientific Library

It is widely accepted that data obtained from primary cells is not only desired, but, most relevant when trying to study physiological interactions. Experimentation in primary cells allows a deeper and

Successful culture of primary cells requires obtaining a maximum yield of functionally viable single cells from whole tissues. The primary factors that affect successful dissociation of whole tissue have

Gene expression is regulated by different mechanisms. One of them is the binding of

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

Tissue culture for Primary cell isolation are often jeopardized by the outgrowth of contaminating fibroblasts. As fibroblasts usually grow at a much faster rate compared to the target

According to the WHO (World Health Organization), early diagnosis of diseases is one of the key factors in the world to save lives and reduce the cost of treatment. Faced with these recommendations, in