Scientific Library

Cell-based assays have become a classic way to monitor cells' reactions to a treatment or a specific stimulus. They involve a reporter construction and a detection system. The classic system is Firefly

As with this painted representation of (surrealist!) DNA, to a certain extent, molecular cloning is an art.

Unfortunately for newcomers, or fortunately for experts, it's required for many downstream applications

Inflammation is involved in many fields including cosmetology, drug discovery (including RNA-based vaccines), and diagnostic index development, as well as being linked to almost every disease such as HIV,

In a recently published study, researchers sought to elucidate if 2 treatments modulated immune response or not, despite the available evidence being conflicting. The method that made the difference and

One of the challenges in R&D is to find robust technologies to develop diagnostic tests. Notably, it could be within a context of asymptomatic infections for which reliable detection of positive specimens

Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient

Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

I just want to briefly outline in this post the power of antibody microarray when it comes to time and cost saving as well as versatility. Whether you want to do multiple profiling or quantitative studies,

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of

The close relation between Severe Acute Respiratory Syndrome (SARS), unpaired adaptive immune responses and overexpression of inflammatory cytokines has been described more than a decade ago (1, 2).
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According to the WHO (World Health Organization), early diagnosis of diseases is one of the key factors in the world to save lives and reduce the cost of treatment. Faced with these recommendations, in