Scientific Library

Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis by Monsel A et al.

During my daily discussions with researchers involved in in vitro iPS and Embryonic Stem cell cultures, I am always amazed by the passion that drives them. However, the more I go in depth with

The complement system helps the ability of antibodies and phagocytic cells to clear pathogens from an organism. It is part of the innate immune system that is not adaptable and does not change over the

In this paper (Role of extracellular calcium and mitochondrial oxygen species in psychosine-induced oligodendrocyte cell death), Voccoli et al. used Human oligodendrocyte MO3.13 cells to study Globoid cell leukodystrophy.

Globoid cell leukodystrophy (GLD) is a metabolic disease caused by mutations in the galactocerebrosidase (GALC) gene. GALC is a lysosomal enzyme whose function is to degrade galacto-lipids, including galactosyl-ceramide and galactosyl-sphingosine (psychosine, PSY). GALC loss of function causes progressive intracellular accumulation of PSY. It is widely held that PSY is the main trigger for the degeneration of myelinating cells and progressive white-matter loss. However, still little is known about the molecular mechanisms by which PSY imparts toxicity. In this paper, the role of calcium dynamics during PSY-induced cell death is addressed. Using the human oligodendrocyte cell line MO3.13, the authors report that cell death by PSY is accompanied by robust

We have been closely following the interesting case of the discontinuation of tobacco acid pyrophosphatase (TAP) and the efforts of the world's RNA biologists to find a suitable alternative. Our previous

The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable

For about 5 years now, there has been a renewed interest for mRNA molecules and their numerous applications. Mature mRNAs can be transfected into cells (ex. in mammalian cells) for

Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of 5 optimized

Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase

StemBeads^® FGF2 is a revolutionary growth factor supplement that offers a more efficient way to grow FGF2 dependent stem cell cultures.

Neurotrophic factors are known to protect neuronal cell death in several neurodegenerative diseases, both in in vitro and in vivo. They play a key role in development, differentiation,

High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE®