Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable

In drug discovery screening campaigns as well as in fundamental research activities, cellular parameters have to be measured and monitored in living cells regularly. Often fluorescent dyes are used to

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of

Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

In a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient

Non-invasive manipulations of intracellular processes have recently appeared as an emerging research field. Experts in the development of biosensors for live cell discovery, Montana Molecular provide an

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System