Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of

Autotaxin (ATX) is a secreted lysophospholipase D involved in the release of the lipid mediator LPA. Many research tools have been developed to study ATX-LPA signaling.  In this post, I invite you

Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient

In a previous post, I introduced Sekisui Xenotech's Research Biobank, which is based on rejections from liver transplantation as opposed to post-mortem tissues, thus offering a guarantee for better tissue

Echelon Biosciences are specialized in the development of novel assays and reagents in Lipid research, with the commitment to innovate and provide the best solutions for researchers. They have recently

Developing new therapeutic agents to boost the immune system and avoid the escape of diseased cells from the natural defenses is the common goal of all researchers in Immunotherapy. To greatly reduce time

The first time a cell biologist discovers that there might be a lipid involved in their favourite pathway, it's a bit like finding a wall across the road (just the same situation as when a lipidomist

Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid...) to resist to physical and mechanical constraints

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System