Scientific Library
Why is the hCMEC/D3 cell line a robust in vitro BBB model?
The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable
Make sure you detect all your Human IFN-alpha
For humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence.  A lot of individual human IFN-α subtypes have been identified and many have different
Next generation RIPA buffer: a new kit for Lipid Raft
RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated
5 multicolour flow cytometry reagents for immunophenotyping
Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of
2 new validated anti-PGP 9.5 antibodies for neuroscience research
Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase
New Zika virus monoclonal antibodies
Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,
New effective anti-Human IFN neutralizing antibody
PBL Assay Sciences offer a new monoclonal antibody to neutralize Human Interferon (IFN) activity,
Human IFN ELISA test: unrivaled sensitivity and global IFN alpha detection
PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha subtypes (41115
New - 4 Unique IFN Neutralizing reagents
PBL Assay Science, the specialists of Interferon related quality reagents, have just released 4 new unique products to be used in neutralization assays. I am pleased to introduce them in this post, in
New: Nipah Virus G Protein and antibodies for developing specific NiV ELISA test
Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient
Establishing meaningful SYBR Green-based qPCR assays
Establishing meaningful SYBR Green-based qPCR assays
SYBR Green qPCR with Standard Curve Protocol
Note from the Author:
This protocol is based on experiences using the StepOnePlusâ„¢ Real-Time PCR System