Antibody pairs have been used for years to detect biomarkers in biological samples. They are a convenient and price-wise alternative to ready-to-use ELISAs, especially when they are used on a routine basis

Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can be used as

What are metabolites?

Microorganisms live in a world of chemical signals. They use small molecular weight compounds, known as metabolites, to regulate their own growth and development, to encourage other

The influenza season in Europe is over... until next year. Influenza remains one of the most important public healthcare problems, especially in risk groups.

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals.

Microbial metabolites are a source of unequalled elements for in vitro assays. Sometimes  neglected, these active molecules naturally provide a large spectrum of structural diversity for Drug discovery

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Small molecules known to modulate biological activies of the K-Ras oncogene are predominantly of synthetic origin (ex. Salirasib). Nevetheless, various publications have recently demontrated the biological

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

If your research interest is focused on the identification of cell signalling pathways and protein phosphorylation profiles, the comprehensive Phospho Explorer Array may be of great help to you, measuring

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System