Scientific Library
Profile 2000 protein targets with the Human L2000 antibody array
RayBiotech , the antibody array specialist, have just released a new, very broad antibody microarray, the L2000 series, for the profiling of 2000 protein targets including metabolic enzymes, structural
Luciferase promoter reporter clones
Cell-based assays, screening for pathway activation or inhibition are classically done with promoter reporter clones expressing Firefly luciferase. Brighter, more stable, more sensitive, more convenient
Screen 500 mouse proteins on a single array
In a previous post, I introduced the G-Series Mouse antibody arrays to profile up to 200 mouse proteins using a combination of 5 antibody arrays on glass slide. This new Mouse L2 L-Series (label based
Proteomics Biostats: get the most out of your array data
High density antibody arrays represent powerful technologies which have opened a new era in functional proteomics. However, when faced with a large magnitude of array data, you must apply comprehensive
shRNA set with improved performances
Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the
Nephropathy research toolbox: from ELISA to Arrays and Primary cells
Nephropathy is a hot research topic, especially in relation to its link with Diabetes (already 6 500 publications so far referenced in pubmed to date for 2019, a third of them in relation to type I or
ORF expressing Lentiviral system
ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell
Accelerate your Biomarker discovery with High Density arrays
While Raybiotech recently released their 2000 target antibody array, a publication from "The Scientist" highlighted the benefit of these High-density multiplexed technology in biomarker discovery. As
Gene knock-out in hard-to-transfect cells
Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target the gene of
Hepatocyte Pellets - why should you make the switch?
You may have heard about the patented CryostaX® format of hepatocyte pellets, but do you know why SEKISUI XenoTech and countless other researchers have made the switch from traditional hepatocyte
ELISA : Quality Control and Performance Evaluation
The ELISA test (acronym of Enzyme Linked ImmunoSorbent Assay) is a test principally used In immunology to detect and/or assay a protein, antigen or antibody in a biological fluid sample . It has many practical
Decoding the Tumor Microenvironment: Advanced Proteomics for Biomarker Discovery & Immunotherapy Insights
Watch an insightful webinar where we delve into the complex network of the Tumor Microenvironment (TME) and explore how advanced multiplex proteomic analysis tools from our partner RayBiotech can unlock