A publication earlier this year by Bárcena et al. shows that angiogenin plays a crucial role in the onset and development of liver cancer (hepatocellular carcinoma or HCC).

Angiogenin was the first

If you're studying human pulmonary function and pathophysiology, you need access to validated, highly characterised, human airway cellular models. A large collection of these cells is available

RIPA buffer is one of the most useful protein extraction buffers and maintains most native structures of proteins. Nevertheless, RIPA buffer is not convenient enough to extract membrane proteins and membrane-associated

Abnova offer a series of new monoclonal research antibodies to support the study of the Zika virus. These are available through tebu-bio across Europe. Let's take a look at these monoclonals,

Gastrointestinal (GI) primary cells represent a powerful approach for the in vitro study of the physiopathology of this unique tissue. Researchers have now identified intimate interactions between GI

Nipah Virus Infection (NiV) is an emerging infectious disease of public health importance in the South-East Asia Region (source: World Health Organization). Along with Hendra virus (HeV), NiV is part

In the field of neurosciences, increasing research is being undertaken on neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as on nerve injury and regeneration. One of the

Working with primary cells can be tricky at times. It's always best to know in advance what essential markers your cell model does express not to waste time and efforts in working with an inappropriate

Producing specialized media for specific primary cell types may be best outsourced to specialized manufacturers in order to obtain optimal results for this type of cell culture, more tricky than standard

Due to their ability to simulate the characteristics of most soft tissues and natural scaffolds, hydrogels are now an attractive material for developing synthetic Extracellular Matrix (ECM) analogs.

Recently,

In a previous post, I introduced a complete panel of ready-to-use specialized media for smooth muscle primary cells culture. Produced by Cell Applications, a GMP certified manufacturer of primary cells

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System