Scientific Library
Why is the hCMEC/D3 cell line a robust in vitro BBB model?
The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects...). In the early phases of drug development, there is a strong need for stable
Antibody purification troubleshooting tips
Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade
3 tips for IHC troubleshooting
The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative
5 multicolour flow cytometry reagents for immunophenotyping
Multicolour fluorochrome reagents have spurred researchers' capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let's take at look at the advantages of
2 new validated anti-PGP 9.5 antibodies for neuroscience research
Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase
Cell-based assays with hPBMCs for cell therapy and drug discovery
Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and
Obtain clear Western Blot and Immunoprecipitation results
Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.
The TrueBlot® reagents
Breast Cancer - Popular validated primary antibodies for research and biomarker discovery
Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated
Anti-Tag antibodies you can rely on: a focus on GFP and RFP
My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing
Lympholyte®: The ideal tool for simple and viable lymphocytes isolation
High quality isolation of lymphocytes is a key step in many in vitro research programs covering immunology, infectious diseases and oncology. To simplify this step, CEDARLANE® have developed an efficient
Immunoassay development: reliable sources of quality reagents
We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we've established