Scientific Library
DyLightâ„¢ will be your routine fluorescent dye
Rockland Immunochemicals have developed a suite of fluorescent dyes (DyLight™) with unique fluorescence intensity and photostability. Here, we take a look at how DyLight™ dyes have all the characteristics
Following your proteins - anti-tag antibodies (I)
When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not
Antibody purification troubleshooting tips
Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade
3 tips for IHC troubleshooting
The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative
Cell-based assays with hPBMCs for cell therapy and drug discovery
Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and
Obtain clear Western Blot and Immunoprecipitation results
Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.
The TrueBlot® reagents
Breast Cancer - Popular validated primary antibodies for research and biomarker discovery
Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated
Easy single-cell isolation with Smart Aliquotor
Single-cell isolation allows genomic and transcriptomic analysis of one individual cell. It is also required to build a monoclonal cell line from rare cell isolation, that could be for example CRISPR-CAS9
Low-cost, high-throughput device for single-cell RNA sequencing
The main challenge in RNA-sequencing workflow is certainly library preparation. When it's question of single-cell isolation and so 10-100 picogram of total RNA, the challenge may become mission impossible...
Anti-Tag antibodies you can rely on: a focus on GFP and RFP
My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing
Immunoassay development: reliable sources of quality reagents
We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we've established