Scientific Library

Small GTP-binding proteins such as RhoA, Rac1, and Cdc42 are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. They bind and hydrolyze

Ras and Rho family members are small G proteins involved in the regulation of actin-dependent cell processes such as motility, growth, and intracellular trafficking. Furthermore, dysfunctions of Ras and

Free radicals and Reactive Oxygen Species (ROS) are highly reactive molecules generated by normal cellular processes, environmental stress, and UV irradiation. ROS react with numerous cellular components

Actin can be stained in living and fixed cells to determine and follow the structure and function of the cytoskeleton. The actin cytoskeleton is a very dynamic and labile structure in the living cell,

In this post, I invite you to discover some state-of-the art methods to measure the activation of the most prominent small G proteins.

In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins,

You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.

Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest

In this post, I'd like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation,

Today, I'd like to give you an overview about methods in actin research with validated R&D products and kits which will allow you to measure binding to actin and effects on the polymerisation dynamics

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras