Scientific Library
Focus on the AKT cell signaling pathway...
AKT (Protein Kinase B or PKB), is a serine/threonine-specific protein kinase with known key involvement in glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.
Recent advances
Flow cytometry intracellular staining
Intracellular Flow Cytometry enables the identification and analysis of signaling and functional markers within cells from samples containing heterogeneous cell populations. Many cell types can
How sample preparation can affect your biomarker studies (I)
In previous posts, we have discussed about whether samples should be pooled, how much (and if) they should be diluted, and how biologicals can affect the final results...and the biomarkers that you find.
Following your proteins - anti-tag antibodies (I)
When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not