Page 5 - Scientific Library

Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and

If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?

Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,

Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing

In previous blogs, I invited you to join me in exploring the relevance of the following pathways:

• B7-1 : CD28, B7-1 : CTLA4
• BLTA:HVEM, CD47:SIRPα
• GITR:GITRL
• CD40:CD40L
• PD-1/PD-L1/PD-L2
• CD137:CD137L

Caspases (cysteine-dependent aspartate-directed proteases) belong to the family of cysteine proteases and are involved in networks controlling cell death (apoptosis and necrosis) and inflammation.

In a previous post, I discussed Caspases as pharmaceutical targets - how to screen for inhibitors?

Today I would like to concentrate on Caspase inhibitors/modulators, which allow for in-depth characterisation

Methylation of DNA plays a crucial role in transcriptional regulation, imprinting, and chromatin

Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such

Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol

Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.

This technology produces first a targeted break in genomic DNA, which can then be exploited to produce

A recent paper by Kojima, M. et al. has found