Page 5 - Scientific Library
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start with
6 Tips for a successful ELISA
ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands
Glycosylation studies in the inflammasome
In a previous post on tumour microenvironment (TME) and glycosylation, we indicated that new tools for studies on glycosylation of proteins were being developed. We are happy to announce that they are
Novel biomarkers in gastric cancer
The word "cancer" groups a series of diseases of different origin and different prognosis, with differentiated molecular mechanisms causing their onset and progression. Hence, discovery of novel biomarkers
Direct access to Biomarker Profiling identification tools
Profiling tools have an increasing interest for identifying new biomarkers. Different tools are available, from classical gene profiling on DNA chips, RT-qPCR arrays, Protein arrays for secretome or
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
Genome Editing in Stem Cells: outsource or do-it-yourself?
Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing
Diluting samples for proteomics – biomarker profiling case studies (I)
To dilute or not to dilute biological samples? And if yes, how much? That's the question!
Following our post on whether samples should be pooled or not when performing studies for Biomarker discovery,
Biomarker profiling: Diluting samples case studies (II)
Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.
Case # 1 - ELISA or simplex assays
Most ELISA manufacturers