Page 4 - Scientific Library
Focus on Actin - G-F Actin ratio measured in cells
In previous posts,  I invited you to look at versatile methods to
- Visualize actin: Focus on Actin staining and visualization
- Measure actin binding: Focus on Actin – Detection of actin binding and
Tubulin and microtubule vizualization in cells
Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells
Blood Urea Nitrogen (BUN) assays - no ammonia interference!
The determination of serum blood urea nitrogen (BUN) is currently the most widely used screening test for the evaluation of kidney function. The test is frequently run along with the serum creatinine test,
A new fast and sensitive PGE2 Immunoassay kit
PGE2 is a potent lipid mediator involved in maintaining homeostasis but also in the promotion of acute inflammation or immune
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen
58 different human RTKs in one phosphorylation array
The Receptor Tyrosine Kinases (RTKs) are an important family of cell-surface receptors transmitting extracellular stimuli (ex. NGF, PDGF, FGF, EGF, Insulin...) into intracellular protein-tyrosine kinase
Versatile measurement of protein binding to microtubules
You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.
See how researchers get good quality publications with arrays
How sample preparation can affect your biomarker studies (II)
In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.
Tip #1
Tubulin polymerisation measurement - made easy!
Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for
Find metastatic biomarkers in osteosarcoma!
Early in 2015, researchers of The University of Queensland Diamantina Institute (Australia) have shown a very sensible approach to the discovery of new biomarkers associated to transition from non-metastatic