Page 3 - Scientific Library
MDR1 activity enhancement reduces senescence markers
During the last International Federation of Societies of Cosmetic Chemists (IFSCC) congress (Paris, Oct. 2014), Hajem N. et al (ALES Groupe) have shown that by enhancing MDR activity of fibroblasts
Physioxia or Hypoxia: what it's all about
Cell culture under oxygen controlled conditions: an improvement towards more predictive results
Oxygen concentration in tissues is a key factor for cell and organ survival. In normal conditions, partial
Actin staining techniques in fixed and living cells
Actin can be stained in living and fixed cells to determine and follow the structure and function of the cytoskeleton. The actin cytoskeleton is a very dynamic and labile structure in the living cell,
Focus on Actin staining and visualization
Actin serves as one of the major cytoskeleton structures. It is a crucial component involved in a plethora of processes in cell biology: stabilizing the cell shape, cell movements (e.g. cell migration)
Discovery of new biomarkers... 3 tips regarding controls
One of the recurrent questions that we get at the Biomarkers team at tebu-bio is on what controls should be included in a given experiment. Either if the experiment is done by researchers in their lab,
Focus on Actin detection and Actin binding proteins
Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes
How sample preparation can affect your biomarker studies (I)
In previous posts, we have discussed about whether samples should be pooled, how much (and if) they should be diluted, and how biologicals can affect the final results...and the biomarkers that you find.
Focus on Actin - G-F Actin ratio measured in cells
In previous posts,  I invited you to look at versatile methods to
- Visualize actin: Focus on Actin staining and visualization
- Measure actin binding: Focus on Actin – Detection of actin binding and
Tubulin and microtubule vizualization in cells
Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen