Page 3 - Scientific Library

In the past, molecular motor proteins like kinesins have been investigated with macromolecular approaches. Recent research on kinesins has been focused on resolving how kinesin is regulated by intramolecular

Small GTP-binding proteins such as RhoA, Rac1, and Cdc42 are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. They bind and hydrolyze

Ras and Rho family members are small G proteins involved in the regulation of actin-dependent cell processes such as motility, growth, and intracellular trafficking. Furthermore, dysfunctions of Ras and

Actin can be stained in living and fixed cells to determine and follow the structure and function of the cytoskeleton. The actin cytoskeleton is a very dynamic and labile structure in the living cell,

Assessing gene transcription as an endpoint for determining induction of drug metabolizing enzymes and transporters has become the “method of choice” in the FDA’s latest draft Guidance for Industry drug

A wide range of in vitro models are used in preclinical drug testing for the investigation of ADME-Tox (Absorption, Distribution, Metabolism, Excretion and oxicity) properties of New Chemical Entities

Actin serves as one of the major cytoskeleton structures. It is a crucial component involved in a plethora of processes in cell biology:  stabilizing the cell shape, cell movements (e.g. cell migration)

Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes

Actin dynamics - i.e. polymerization of G-actin to form filamentous F-actin or de-polymerization of F-actin - is a fundamental

In previous posts,  I invited you to look at versatile methods to

• Visualize actin: Focus on Actin staining and visualization
• Measure actin binding: Focus on Actin – Detection of actin binding and

Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells

In this post, I invite you to discover some state-of-the art methods to measure the activation of the most prominent small G proteins. In future posts, we'll