Page 3 - Scientific Library
miRNA mediates gene expression through HDL particles
A recent study published in Nature Communications is the first to report how a mature microRNA (miR-223) actively controls the gene expression of a cell in which it was not originally transcribed. High
Tumour microenvironment and miRNA biomarkers
In previous posts, we have seen the role of inflammation and glycosylation in the tumour microenvironment (TME). All these are mainly factors at the protein level causing the tumour cells to evade the
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start with
The TRPV1 Pain Receptor activates T cells
The Transient Receptor Potential (TRP) ion channel family participates in numerous functions of the Nervous System. A recent publication tends to indicate that TRP might also be of interest for therapeutical
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
5 reasons to prefer custom rec. protein production to commercial
Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so there shouldn't be any need to look for alternatives
Genome Editing in Stem Cells: outsource or do-it-yourself?
Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing
How to manipulate and measure Autophagy?
The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes - which was held in London in February 1963. In 1974 he was honoured with the Nobel price in
Ferroptosis - and the way to inhibit it
A lot of compounds are known and available to modulate (inhibit or activate) the most prominent mode of programmed cell death - Apoptosis. In a recent post, I summarized methods to detect early Apoptosis
Differentiate between Apoptosis, Necroptosis, Autophagy & Ferroptosis
Cell death can be caused by external factors such as infection or trauma - a process which is call necrosis. On the other hand, cell death can be mediated by intracellular programs - in this cases we talk