Page 2 - Scientific Library

Rockland Immunochemicals have developed a suite of fluorescent dyes (DyLight™) with unique fluorescence intensity and photostability. Here, we take a look at how DyLight™ dyes have all the characteristics

In this post, I invite you to discover some state-of-the art methods to measure the activation of the most prominent small G proteins. In future posts, we'll

In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen

When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not

You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.

In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.

Tip #1

Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for

Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade

The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative

The unprecedented increase in antibiotic-resistant pathogens and lack of

In this post, I'd like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation,