Page 2 - Scientific Library
Sensitive ELISA for Oxytocin quantification
The neuropeptides, Oxytocin and Vasopressin, were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955.
Focus on Actin - G-F Actin ratio measured in cells
In previous posts,  I invited you to look at versatile methods to
- Visualize actin: Focus on Actin staining and visualization
- Measure actin binding: Focus on Actin – Detection of actin binding and
Tubulin and microtubule vizualization in cells
Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen
Versatile measurement of protein binding to microtubules
You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.
How sample preparation can affect your biomarker studies (II)
In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.
Tip #1
Tubulin polymerisation measurement - made easy!
Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for
Tubulin:Microtubule ratio measured in cells
Reactive Oxygen Species (ROS) and related assay kits
Reactive Oxygen Species (ROS) are reactive molecules and free radicals derived from molecular oxygen involved in cellular homeostasis. An excess of ROS production (e.g. exposure to environmental stress