Page 2 - Scientific Library
Tumour microenvironment and miRNA biomarkers
In previous posts, we have seen the role of inflammation and glycosylation in the tumour microenvironment (TME). All these are mainly factors at the protein level causing the tumour cells to evade the
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start with
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,
Genome Editing in Stem Cells: outsource or do-it-yourself?
Many researchers are facing a dilemma: they want to set up a CRISPR genome editing project but they can't decide which cell line to use for genome editing. Even some of the most cost-effective genome editing
Using CRISPR to knockout an essential gene
Genome editing technology enabled by CRISPR and TALEN has become mainstream. Most cell biology labs are engaged in projects to create custom cell lines with knock-outs and knock-ins, and companies such
Kit-Free Site Directed Mutagenesis Protocol
Molecular biologists are familiar with the QuikChange® Site-Directed Mutagenesis Kit that allows rapid intoduction of a point mutant into a plasmid/vector/mammalian expression construct. Briefly, the protocol
CRISPR genome editing: which cell line to choose?
Many labs have adopted the CRISPR genome editing technology to make knock-out and knock-in cell lines.
This technology produces first a targeted break in genomic DNA, which can then be exploited to produce
miRNAs: potent biomarkers in cancer research?
A recent paper by Kojima, M. et al. has found
All GeneCopoeia products and services available from tebu-bio
GeneCopoeia brand products include any possible DNA construct/plasmid in addition to an ever-growing offer of associated products and services. Here is a quick overview of the offer:
If you search for
Oxygen and miRNAs in Cosmetology and Dermatology
A recent review by Nadim et al. casts some light on a Cosmetology and Dermatology, where circulating biomarkers, though studied to a certain extent so far, are yet unknown for many skin models. (1)Â