Page 2 - Scientific Library

What are metabolites?

Microorganisms live in a world of chemical signals. They use small molecular weight compounds, known as metabolites, to regulate their own growth and development, to encourage other

Actin serves as one of the major cytoskeleton structures. It is a crucial component involved in a plethora of processes in cell biology:  stabilizing the cell shape, cell movements (e.g. cell migration)

Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes

Actin dynamics - i.e. polymerization of G-actin to form filamentous F-actin or de-polymerization of F-actin - is a fundamental

In previous posts,  I invited you to look at versatile methods to

• Visualize actin: Focus on Actin staining and visualization
• Measure actin binding: Focus on Actin – Detection of actin binding and

Recently, a we published a series of posts focusing on research tools aiming at studying actin through biochemical assays and in fixed and living cells

In this post, I invite you to discover some state-of-the art methods to measure the activation of the most prominent small G proteins. In future posts, we'll

In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen

You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.

In a previous post, we looked at the importance of sample preparation methods depending on the biomarker being studied (e.g. MMPs, cytokines). Today, let's focus on cell / tissue lysates.

Tip #1

Microbial metabolites are a source of unequalled elements for in vitro assays. Sometimes  neglected, these active molecules naturally provide a large spectrum of structural diversity for Drug discovery

Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for