Scientific Library
FAQs on feeder cells for optimal human iPSC culture
Human Newborn Foreskin Fibroblasts (NuFF cells) are genuine feeder cells for Stem cell research. Mitotically inactive NuFF cells (by irradiation or Mitomycin C chemical treatment) can efficiently maintain
Focus on the Hypoxia Regulation Mechanism
The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary
ATM or DNA-PKcs inhibitors synthetically lethal in BRCA- cells
Several lines of evidence show that synthetic lethal interactions targeting DNA repair pathways may have clinical applications in cancer therapy. In a recent paper, Albarakati et al. demonstrate that
MDR1 activity enhancement reduces senescence markers
During the last International Federation of Societies of Cosmetic Chemists (IFSCC) congress (Paris, Oct. 2014), Hajem N. et al (ALES Groupe) have shown that by enhancing MDR activity of fibroblasts
Physioxia or Hypoxia: what it's all about
Cell culture under oxygen controlled conditions: an improvement towards more predictive results
Oxygen concentration in tissues is a key factor for cell and organ survival. In normal conditions, partial
Discovery of new biomarkers... 3 tips regarding controls
One of the recurrent questions that we get at the Biomarkers team at tebu-bio is on what controls should be included in a given experiment. Either if the experiment is done by researchers in their lab,
Why you should outsource your plasmid preps
Plasmid preps are to science what salt is to cooking. They're so common that nobody notices, but nothing can be done without them. There is no mention of this step in any published paper, nobody mentions
Why you should outsource your production of proteins - both stable or unstable
Whether your protein is simple to produce, or complex and requires more development, in any case, outsourcing your protein expression, purification and production is a very viable option for your projects.
New - you know CRISPR, now it's Prime Editing
This month, a new leap forward in gene editing has been published in Nature under the title 'Search-and-replace genome editing without double-strand breaks or donor DNA'. DOI: 10.1038/s41586-019-1711-4.
How to make efficient Lipid Nanoparticles for RNA delivery
How to make efficient Lipid Nanoparticles for RNA delivery
Even if RNA-based therapeutics have been under development for over 30 years, it was the COVID-19 pandemic that brought them into the limelight,
What is the future of mRNA based therapeutics, and their main challenges?
mRNA is a ribonucleic acid used by cells as an intermediate carrier of the genetic information contained in the DNA to synthesize proteins, and corresponds to the copy of one of the two strands of DNA.
Why replace fluorescent markers with lanthanide nanoparticles to improve detection?
According to the WHO (World Health Organization), early diagnosis of diseases is one of the key factors in the world to save lives and reduce the cost of treatment. Faced with these recommendations, in