Protein phosphorylation analysis is  more and more used for cell signaling studies. This post-translational modification regulates the function of numerous proteins in both physiological and disease-related

Establishing meaningful SYBR Green-based qPCR assays

SYBR Green qPCR with Standard Curve Protocol

Note from the Author:
This protocol is based on experiences using the StepOnePlus™ Real-Time PCR System