Scientific Library

ELISA are immunoassays widely used in biomarker detection and validation. They have been used in research and clinical settings for more than 40 years, and they allow to quantify, in a simple way, thousands

Classically, it was known that CD4⁺ T cells, upon activation and expansion, develop into different T helper cell subsets with different cytokine profiles and distinct effector functions to effect

Immunoediting of cytokine signals provides another area of pathways which tumours utilise to evade and potentially escape from immunological targeting. This post aims at shedding some light into the Tumour

For humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence.  A lot of individual human IFN-α subtypes have been identified and many have

With more than 170,000 publications in 2016, inflammation remains a "hot" topic in today's Life Science research (source: Google Scholar). Interestingly, the ELISA test is still one of the most popular

PBL assay specializes in the development of IFN related research Tools. One of their most appreciated assay kit by the IFN research community is the ELISA test kit for all Human IFN Alpha

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

Interestingly, the experimental design is simple and is based on 3 steps:

1. Plate
2. Treat and fix your cells of interest (adherent or in suspension)
3. Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)

These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily

As is already known, multiple families of small GTPases (Ras, Arf and Rho families) make up the Ras

Tetramethylbenzidine (TMB) is a classic substrate for peroxidase activity that is used for microwell plate analysis such as an ELISA test. A quantitative color change from yellow to

The ELISA test (acronym of Enzyme Linked ImmunoSorbent Assay) is a test principally used In immunology to detect and/or assay a protein, antigen or antibody in a biological fluid sample . It has many practical