Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bio-luminescence (Fig.
Page 3 - Scientific Library
How to monitor mitophagy in mammalian cells
Mitochondria keep cells "healthy" in their environment. In this post, we'll take a look at a new fluorescent assay for the monitoring of mitophagy, a process aimed at clearing damaged or superfluous mitochondria
LipiDye fluorescent probe for lipid droplet monitoring in live cells
LipiDye lipid droplet Green* is a new highly sensitive fluorescent probe for detecting lipid droplets in live or fixed cells. In this, let's review the characteristics of this dye which is compatible
How to activate or inhibit autophagy?
The term Autophagy was introduced by Christian de Duve during the Ciba Foundation Symposium on Lysosomes – which was held in London in February 1963. In 1974 he was honoured with
Ppc-1 small molecule: a new mitochondria uncoupling agent
Ppc-1 is a novel small molecule derived from cellular slime mould. In this post, I'd like to introduce the characteristics of this compound for your in vitro mitochondrial research.
Fluorescence microscopy: how to improve your Live Cell Imaging
For more than 2 years now, the Silicon Rhodamine-like (SiR) technology has allowed the live cell imaging field with fluorescence microscopy to evolve significantly.
Fluorescent
Stauprimide inhibits c-myc transcription in cancer cells
Stauprimide is known to prime Embryonic Stem Cells (ESC) by targeting the c-Myc-activating transcription factor NME2. Its mechanism of action is linked to the inhibition of the nuclear localization
Biomolecule conjugation - how to biotinylate with reproducible results?
Biomolecule conjugation is critical to many applications such as bead-based assays, ELISA and chromatography by affinity. Unfortunately the classical methods are known to be tedious and with low
Bioactive neuronal lipid mediators: a focus on Synaptamide
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular
GPR17 and Remyelination: activators and antagonists
The absence of mature oligodendrocytes for the repair of demyelination lesions is a critical factor in the pathology of Multiple Sclerosis (MS), and other debilitating CNS disorders
Improve MS-based biomarker determination with immunocapture
During the past decade, mass spectrometry, notably LC/MS, has become a major approach to identify and quantify proteins in patient samples. It includes analysis before treatments to find the proper biomarkers