Scientific Library
2 assays to assess GEF and GAP activity
In my previous post Small GTPases: Measuring small G protein activation I looked at state-of the-art methods for measuring the activation of small G proteins, such as RhoA, Rac1, Cdc42, and the proto-oncogen
Versatile measurement of protein binding to microtubules
You suspect your protein binds to microtubules? That it might stabilize or destabilize these filamentous structures? Then this post is here to help you to find a meaningful assay to validate your assumption.
Tubulin polymerisation measurement - made easy!
Recently, I issued a post about a method which allows measuring microtuble binding capabilities of proteins of interest. Today, I invite you to look at methods for
Tubulin:Microtubule ratio measured in cells
3 tips for IHC troubleshooting
The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative
Post-Translational Modifications (PTM) of Tubulin
In this post, I'd like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation,
3 functional assays to investigate actin dynamics
Today, I'd like to give you an overview about methods in actin research with validated R&DÂ products and kits which will allow you to measure binding to actin and effects on the polymerisation dynamics
Obtain clear Western Blot and Immunoprecipitation results
Generating high-quality Immunoprecipitation & Western Blot (IP & WB) data has never been easier. See how you can easily achieve this in our Tips & Tricks section to get clean WB-IP.
The TrueBlot® reagents
Breast Cancer - Popular validated primary antibodies for research and biomarker discovery
Use of validated antibodies is primordial for Breast Cancer research and related biomarker discovery. Here, we'll review the main biomarkers involved in this disease and introduce the most popular validated
Cell signalling: How to easily detect mono- and poly-Ub target proteins
Anti-Tag antibodies you can rely on: a focus on GFP and RFP
My colleague Ana introduced in a previous post (3 years ago already!) a selection of the most popular anti-tag antibodies among the research community. I must say that they remain extremely popular, showing
Immunoassay development: reliable sources of quality reagents
We understand how crucial it is to have access to sustainable, quality sources of reagents when developing and/or producing the same immunoassays over long periods of times. Over the years, we've established