Page 11 - Scientific Library
Biomarkers of micronutrient malnutrition
Unfortunately, and still in the XXIst century, micronutrient (MN) malnutrition is a problem in all countries, in all segments of society. While part of the world starves, another part is obese. And a balanced
Immunotherapy Screening - IDO pathway
In previous blogs, I invited you to join me in exploring the relevance of the following pathways:
- B7-1 : CD28, B7-1 : CTLA4
- BLTA:HVEM, CD47:SIRPα
- GITR:GITRL
- CD40:CD40L
- PD-1/PD-L1/PD-L2
- CD137:CD137L
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Biomarker profiling: Diluting samples case studies (II)
Back to dilution of samples. After our post on whether samples should be diluted or not, let's discuss today about how much they should be diluted.
Case # 1 - ELISA or simplex assays
Most ELISA manufacturers
Put the odds in your favour for your STED microsopy images
Stimulated emission depletion (STED) microscopy is an accurate process allowing super resolutive cell imaging. STED microscopy overcomes the limits of standard confocal laser scanning microscopes. In
Discovery of functional serum biomarkers
Lung cancer is one of the most common malignancies, and the leading cause of cancer-related fatality. Current diagnostic practices for common cancers rely heavily on imaging technologies. These
New biomarkers in periodontal disease
Periodontitis, also generally called gum disease or periodontal disease, begins with bacterial growth in the mouth and may end, if not properly treated, with tooth loss due to destruction of the tissue
How to measure Glycosaminoglycans and Proteoglycans?
Glycosaminoglycans (or mucopolysaccharides) are long un-branched polysaccherides which consist of disaccharide repeats. The repeats usually (with the exception of Keratan sulfate) consist of on amino sugar
Which Antibodies for Epigenetics?
The nucleosome core particle is the fundamental structural unit of the eukaryotic genome. It consists of a histone octamer composed of two H2A-H2B dimers and a H3-H4 tetramer wrapped by ~146 base pairs
mTOR revisited
The PI3K/Akt/mTOR pathway is an intracellular signaling pathway important in regulating the cell cycle. It is directly related to cellular quiescence, proliferation, cancer, and
Caspases as pharmaceutical targets - screening for inhibitors?
Caspases (cysteine-dependent aspartate-directed proteases) belong to the family of cysteine proteases and are involved in networks controlling cell death (apoptosis and necrosis) and inflammation.
Ferroptosis - and the way to inhibit it
A lot of compounds are known and available to modulate (inhibit or activate) the most prominent mode of programmed cell death - Apoptosis. In a recent post, I summarized methods to detect early Apoptosis
5 Tips for Flow Cytometry
Flow cytometry, even if somehow a "classical" technique, is still very valuable in Immunology. Uses of flow cytometry vary from cell identification & sorting based on membrane molecules, characterisation