Page 12 - Scientific Library

The nucleosome core particle is the fundamental structural unit of the eukaryotic genome. It consists of a histone octamer composed of two H2A-H2B dimers and a H3-H4 tetramer wrapped by ~146 base pairs

The PI3K/Akt/mTOR pathway is an intracellular signaling pathway important in regulating the cell cycle. It is directly related to cellular quiescence, proliferation, cancer, and

Caspases (cysteine-dependent aspartate-directed proteases) belong to the family of cysteine proteases and are involved in networks controlling cell death (apoptosis and necrosis) and inflammation.

A lot of compounds are known and available to modulate (inhibit or activate) the most prominent mode of programmed cell death - Apoptosis. In a recent post, I summarized methods to detect early Apoptosis

Flow cytometry, even if somehow a "classical" technique, is still very valuable in Immunology. Uses of flow cytometry vary from cell identification & sorting based on membrane molecules, characterisation

Cell death can be caused by external factors such as infection or trauma - a process which is call necrosis. On the other hand, cell death can be mediated by intracellular programs - in this cases we talk

ELISAs are widely used in biomarker-related studies, especially when a high number of samples is involved, and only a few biomarkers need to be quantified. That said, incubations can be somehow cumbersome,

In a previous post, I discussed Caspases as pharmaceutical targets - how to screen for inhibitors?

Today I would like to concentrate on Caspase inhibitors/modulators, which allow for in-depth characterisation

Fibrinogen is the main protein of the blood coagulation system. It consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. The process of blood coagulation results

Epothilone B is a bioactive microtubule-stabilizing small molecule. This blood-brain barrier permeable coumpound belongs to the most popular potent cytoskeletal modeling molecules for in vitro cell based

Ubiquitination is a common post translational protein modification that occurs through an isopeptide linkage between the C-terminus of ubiquitin and the ε-amino group of a lysine residue on the target

Intracellular Flow Cytometry enables the identification and analysis of signaling and functional markers within cells from samples containing heterogeneous cell populations. Many cell types can