Page 4 - Scientific Library
microRNAs: Push and observe - miRNA overexpression
In this third post of the miRNA-related series, let's take a look at miRNA overexpression.
miRNA mimics
Short RNAs are very prone to enzymatic degradation. Novel chemical technologies now allow RNA stabilization
microRNAs: Inhibiting the inhibitors - Antagomirs
Following on in our series on microRNAs (miRNA - Keys to open cell biology’s secret chamber) , in this post, we'll be taking a look at Antagomirs (which are also called anti-miRs or blockmirs). These
microRNAs: Target detection
Leading on from my previous posts (see below) exploring microRNA detection, overexpression and inhibition, this time, let's spend a moment on target detection.
miTarget databases
There are many online
Focus on the Hypoxia Regulation Mechanism
The cellular and physiological effects resulting from hypoxia-dependent networks have been clearly shown to impact a number of human pathological states, including ischemic disease, diabetes, pulmonary
Gateway® cloning technology: Is it as easy as they say?
Molecular biology experts have been telling us for a while that the Gateway® cloning vectors are easy-to-use, saving them significant time and effort.
Basically, the technology allows you to start with
3 Reasons to use fresh TLR9 agonists
As part of the innate immune response, Toll-like receptor 9 recognizes unmethylated CpG nucleotide sequences, which are very common in viruses and prokaryotes and more rare in vetebrates. Scientists
AAV - advantages for Gene delivery in vivo
Adenoassociated viruses (AAV) are small (20-30 nm), ssDNA, non-enveloped and replication-defective viruses that infect human and other primate species, but which, up to now, have not been reported as being
What's new in qPCR?
We know molecular biologists spent a lot of time setting up their qPCR protocols and aren't about to change the way they do things. We also know they are technically savvy and like keeping up
Next generation of lentiviral particles
Lentiviruses are robust tools for cell line engineering and development. As opposed to models that only infect dividing cells, lentiviral vectors are ideal for long term gene delivery into dividing and
Exosomes as a trigger for signaling
Extracellular vesicles in general, or exosomes in particular, are becoming a hot topic for research, especially in cancer. They seem to have different roles in tumour progression or can be used as
Adenovirus: weapons of mass transduction for gene delivery
Adenovirus (AV) are non-enveloped and non-integrative icosaedral viruses. Recombinant AV (genetically modified with respect to the wild-type in order to lose their capacity of replication) are widely used
Alternatives to the "mega-brands" of molecular biology
If you don't buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?
Let me guess... you might use those blue and red boxed Qiagen® kits for DNA and RNA purification,