ClickTech EdU T Cell Proliferation Kit 647 for Flow Cytometr - 1 Kit

All EdU based proliferation kits on the market are patented by our company. This click assay has been optimized for the detection of T cell proliferation. Of utmost importance in immunology research is the activation of T cells. This is normally done by BrdU assays and in special applications by fluorescent cell tracing reagents. The used BrdU assays have several limitations as it is time consuming (4 h) and it requires harsh, denaturing conditions of tissue to allow anti-BrdU antibodies to reach the genomic DNA. Additionally the low sensitivity requires a high number of target cells. The indirect fluorescent cell tracing reagent method depends on long incubation time (4 – 7 days) and in higher concentration shows cytotoxicity. The EdU assay is quicker (1 h), simpler, and highly sensitive by the bio orthogonal CuAAC reaction. Therefore, EdU is a superior sensitive alternative to BrdU and the dilution methods, is less cytotoxic and allows improved detection of e.g. interferon gamma r