CBX1 Inhibitor Screening Assay Kit - 384 rxns.

The CBX1 Inhibitor Screening Assay Kit is designed to measure the inhibition of CBX1 binding to its substrate. The kit comes in a convenient AlphaLISA® format, with enough biotinylated peptide substrate, assay buffer, detection buffer and purified GST-tagged CBX1 chromodomain to perform a total of 384 enzyme reactions. The key to the kit is the specific binding of the CBX1 chromodomain to the methylated-peptide substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CBX1 and an inhibitor of choice is incubated with the biotinylated substrate for one hour. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.