Autotaxin Activity Assay - EA KIT

The Autotaxin Activity Assay gives a quantitative measurement of autotaxin (ATX) activity in biological samples by measuring changes in fluorescence over time.Sample Volume: 400 µL / sample (50 µL per well 8 wells per sample)Sample Number: 10 samples (in duplicate) / 96 well assaySample Type: Currently this kit has been tested for the quantification of autotaxin activity in serum plasma ovarian ascites and cell culture media.Product BackgroundThe Autotaxin Activity Assay developed by Echelon Biosciences Inc. utilizes the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore’s fluorescence however once autotaxin cleaves FS-3 the fluorophore becomes liberated from the quencher resulting in increased fluorescence.This assay gives a quantitative measurement of autotaxin activity in biological samples by measuring changes in fluorescence over time