Signal-Seeker® SUMOylation Detection Kit (10 assays) - KIT 10 assays

This kit includes all reagents—controls and inhibitors—to confidently detect SUMOylation of your target protein using a reliable IP/Western blot-based assay.

Applications

• Detect endogenous SUMO2/3 modification in your protein of interest (POI)
• Discover novel regulatory mechanisms for your POI
• Confirm SUMOylation data generated through the transfected POI
• Confirm SUMOylation data generated through proteomic approaches
• Discover novel biomarkers

Material

Kit contents (10 - 30 assays)

• BlastR® lysis buffer
• BlastR® dilution buffer
• BlastR-2® wash buffer
• De-ubiquitination/SUMOylation inhibitor
• Protease inhibitor cocktail
• SUMO2/3 affinity beads
• Control beads
• Anti-SUMO2/3-HRP antibody
• Precision Red™ advanced protein assay reagent
• Bead elution buffer
• DMSO
• Chemiluminescent detection reagents
• Spin column collection tubes
• BlastR® filters

Equipment & materials required

• Cell line or tissue
• Cell scrapers
• Liquid nitrogen for snap freezing lysates
• SDS-PAGE & Western blot equipment
• Primary antibody to POI
• Secondary antibody

Biological Activity Assay

The Signal-Seeker® SUMO 2/3 detection kit uses affinity beads to enrich SUMOylation 2/3 modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard Western blot procedures, and the modified protein of interest is detected by the end-user using their primary antibody.

Key characteristics

• Sensitivity: Demonstrated to detect endogenous SUMO 2/3 modification in multiple target POI
• Input requirements: 200 µg – 1 mg of lysate
• Timeframe: Approximately 10 hours, typically performed over two days