pUC19 Low, Medium, High Methylated DNA Set (50 ng per vial w - 4 vials

The pUC19 Methylated DNA Control Set includes three vials of lambda DNA, each precisely methylated to represent low, medium, and high methylation levels, with each vial containing 50 ng of DNA. This set also comes with a set of specifically designed primers, allowing researchers to directly assess and optimize methylation assays. Each DNA sample is in vitro methylated at CpG sites to achieve targeted methylation levels, making this control set an invaluable tool for both quantitative and qualitative DNA methylation analyses. The applications include: Assay Calibration: The inclusion of low, medium, and high methylated DNA, along with the specifically designed primers, enables precise calibration of methylation assays. This supports accurate quantitative analysis across a range of methylation states. Spike-in Controls: Ideal for use as spike-in controls, these methylation levels can be added within experimental reactions to assess bisulfite conversion efficiency and verify assay perfor