MicroElute® Cycle-Pure & Gel Extraction Kit - 5 - Preps

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA underoptimized conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer. The MicroElute® Clean Up system, designed for rapid DNA clean up, includes: • MicroElute® Cycle-Pure – for direct purification of double or single stranded PCR products (100 bp – 10 kb) from amplification reactions• MicroElute® Gel Extraction – for extraction of DNA fragments from standard, or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer
Product Details
Downstream application: Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 10-15 µL
Starting material: Agrose gel slice
Starting amount: Up to 10 µg DNA
DNA recovered: >90% recovery, 70 bp to 20 kb
Processing mode: Manual, centrifugation/vacuum
Throughput: 1
DNA binding technology: Silica MicroElute spin column
Binding capacity: 10 μg
Processing time: <15 minutes
Special note: Low elution volume