Mag-Bind® SeqDTR - 5 - mL

Mag-Bind® SeqDTR is designed to effectively and reliably remove unincorporated terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads to eliminate excess nucleotides, primers, and small, non-targeted amplification products such as primer dimers. Sequencing products are mixed with the Mag-Bind SeqDTR magnetic particles, which selectively bind DNA. Two rapid wash steps eliminate trace contaminants such as nucleotides and primers to reduce background signal and, therefore, achieve higher QV scores. The high sensitivity of Mag-Bind SeqDTR’s binding ability allows for decreased concentrations of BigDye® chemistry to be used and longer continuous read lengths to be achieved. Mag-Bind SeqDTR can be processed in 96- and 384-well formats and is compatible with many liquid handling instruments, including Hamilton Microlab® STAR™ & STARlet™, Beckman Coulter Biomek® FX & NX, and Tecan Evo instruments. Up to 4 plates can be run in a 96-well format in less than 25 minutes.
Product Details
Downstream application: Sanger Sequencing, Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume: 40 µL of Elution Buffer for 96-well plate protocol. 15-20 µL of Elution Buffer for 384-well plate protocol.
Starting material: ABI Big Dye Chemistry Cycle Sequencing Reactions
Starting amount: 5 – 20 µL
DNA recovery: Sequencing reads start at 40 nt
Processing mode: Automated; Manual
Throughput: 96-384 samples per run
DNA binding technology: Magnetic beads
Binding capacity: Scalable
Storage: 2°C – 8°C